EIF3E Polyclonal antibody
EIF3E Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11352-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11352-1-AP targets EIF3E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EIF3E fusion protein Ag1856 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 3, subunit E |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 48-52 kDa |
GenBank accession number | BC000734 |
Gene symbol | EIF3E |
Gene ID (NCBI) | 3646 |
RRID | AB_2277716 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for EIF3E antibody 11352-1-AP | Download protocol |
IHC protocol for EIF3E antibody 11352-1-AP | Download protocol |
IF protocol for EIF3E antibody 11352-1-AP | Download protocol |
IP protocol for EIF3E antibody 11352-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |