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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3E Monoclonal antibody, PBS Only

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C8

Cat no : 67095-1-PBS

Synonyms

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48, 1C6C8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3E Monoclonal antibody (67095-1-Ig)

    WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF3E Monoclonal antibody (67095-1-Ig)

    IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3E Monoclonal antibody (67095-1-Ig)

    IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67095-1-PBS targets EIF3E in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF3E fusion protein Ag28523 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight 48 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC000734
Gene symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.