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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3M Monoclonal antibody (66025-1-Ig)

    WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3M Monoclonal antibody (66025-1-Ig)

    WB analysis of HeLa using 66025-1-Ig (same clone as 66025-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EIF3M Monoclonal antibody (66025-1-Ig)

    WB analysis of HepG2 using 66025-1-Ig (same clone as 66025-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using EIF3M Monoclonal antibody (66025-1-Ig)

    WB analysis of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3M Monoclonal antibody (66025-1-Ig)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3M Monoclonal antibody (66025-1-Ig)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EIF3M Monoclonal antibody (66025-1-Ig)

    IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EIF3M Monoclonal antibody (66025-1-Ig)

    IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EIF3M Monoclonal antibody (66025-1-Ig)

    IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF3M antibody (66025-1-Ig, Clone: 2A12B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EIF3M Monoclonal antibody (66025-1-Ig)

    IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody 66025-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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EIF3M Monoclonal antibody, PBS Only

EIF3M Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66025-1-PBS
Clone No.2A12B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PCID1, HFLB5, hfl B5, GA17, EIF3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66025-1-PBS targets EIF3M in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF3M fusion protein Ag17965 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit M
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 35-43 kDa
GenBank accession numberBC019103
Gene Symbol EIF3M
Gene ID (NCBI) 10480
RRIDAB_11042448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L2H7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.