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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4A2 Polyclonal antibody

EIF4A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16119-1-AP

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4A2 Polyclonal antibody (16119-1-AP)

    WB analysis using 16119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16119-1-AP (EIF4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF4A2 Polyclonal antibody (16119-1-AP)

    WB analysis using 16119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16119-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using EIF4A2 Polyclonal antibody (16119-1-AP)

    IHC staining of mouse skeletal muscle using 16119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16119-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using EIF4A2 Polyclonal antibody (16119-1-AP)

    IHC staining of mouse skeletal muscle using 16119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16119-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4A2 Polyclonal antibody (16119-1-AP)

    IF Staining of HepG2 using 16119-1-AP

    Immunofluorescent analysis of HepG2 cells, using EIF4A2 antibody 16119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4A2 Polyclonal antibody (16119-1-AP)

    IF Staining of HepG2 using 16119-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF4A2 antibody (16119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, PC-3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16119-1-AP targets EIF4A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9070 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated molecular weight 408 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012547
Gene symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2097376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Protocols

Product Specific Protocols
WB protocol for EIF4A2 antibody 16119-1-APDownload protocol
IHC protocol for EIF4A2 antibody 16119-1-APDownload protocol
IF protocol for EIF4A2 antibody 16119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols