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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4A3 Polyclonal antibody, PBS Only

EIF4A3 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10463-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of mouse liver using 10463-1-AP (same clone as 10463-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of human lung using 10463-1-AP (same clone as 10463-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of Raji using 10463-1-AP (same clone as 10463-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10463-1-PBS targets EIF4A3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4A3 fusion protein Ag0733 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 3
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC004386
Gene symbol EIF4A3
Gene ID (NCBI) 9775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions