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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4A3 Polyclonal antibody, PBS Only

EIF4A3 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10463-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of human lung using 10463-1-AP (same clone as 10463-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of mouse liver using 10463-1-AP (same clone as 10463-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of Raji using 10463-1-AP (same clone as 10463-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EIF4A3 Polyclonal antibody (10463-1-AP)

    IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10463-1-PBS targets EIF4A3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4A3 fusion protein Ag0733 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 3
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC004386
Gene symbol EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2097394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions