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  • Featured Product
  • KD/KO Validated

EIF4A3 Polyclonal antibody

EIF4A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 17504-1-AP

Synonyms

EC:3.6.4.13, DEAD box protein 48, DDX48, DDX 48, ATP-dependent RNA helicase eIF4A-3

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of A549 using 17504-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis using 17504-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of mouse thymus using 17504-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of mouse thymus using 17504-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of A549 using 17504-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of mouse heart using 17504-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of mouse lung using 17504-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of HEK-293 using 17504-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of mouse liver using 17504-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of HeLa using 17504-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    WB analysis of MCF-7 using 17504-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IP experiment of mouse heart using 17504-1-AP

    IP result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human kidney using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human testis using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human testis using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human brain using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human brain using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human spleen using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human spleen using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human lung using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human lung using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human ovary using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human ovary using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human heart using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human heart using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human kidney using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human kidney using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF4A3 Polyclonal antibody (17504-1-AP)

    IHC staining of human kidney using 17504-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EIF4A3 Polyclonal antibody (17504-1-AP)

    IF Staining of MCF-7 using 17504-1-AP

    Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA549 cells, mouse thymus tissue, mouse heart tissue, mouse lung tissue, HEK-293 cells, mouse liver tissue, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17504-1-AP targets EIF4A3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4A3 fusion protein Ag11701 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 3
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC003662
Gene symbol EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2097393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Protocols

Product Specific Protocols
WB protocol for EIF4A3 antibody 17504-1-APDownload protocol
IHC protocol for EIF4A3 antibody 17504-1-APDownload protocol
IF protocol for EIF4A3 antibody 17504-1-APDownload protocol
IP protocol for EIF4A3 antibody 17504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Hematol Oncol

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation

Authors - Meilin Xue
View Article
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
View Article

Cell Res

YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.

Authors - Hailing Shi
View Article
humanIF,WB

Cell Death Differ

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Authors - Despoina Sakellariou
  • KD Validated
View Article
humanWB

Mol Cancer

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.

Authors - Huizhe Wu
View Article
humanWB

J Nanobiotechnology

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

Authors - Xiao Liu
View Article