EIF4E2 Polyclonal antibody

Name: EIF4E2 Polyclonal antibody
Brand: Proteintech
SKU: 12227-1-AP

EIF4E2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12227-1-AP

Synonyms

4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of HeLa using 12227-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12227-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12227-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12227-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12227-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 12227-1-AP
IP result of anti-EIF4E2 (IP:12227-1-AP, 4ug; Detection:12227-1-AP 1:500) with MCF-7 cells lysate 1280ug.
IHC staining of human breast cancer using 12227-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 12227-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12227-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 12227-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 12227-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, mouse kidney tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
RIP	See 1 publications below

Product Information

12227-1-AP targets EIF4E2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF4E2 fusion protein Ag2864 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 4E family member 2
Calculated molecular weight	245 aa, 28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC021690
Gene symbol	EIF4E2
Gene ID (NCBI)	9470
RRID	AB_10642945
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF4E2 antibody 12227-1-AP	Download protocol
IHC protocol for EIF4E2 antibody 12227-1-AP	Download protocol
IP protocol for EIF4E2 antibody 12227-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis Authors - Ji Geng View Article
human	WB,RIP	Cell Death Dis α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation Authors - Hao Wang View Article
human	WB	iScience The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex. Authors - Limei Zou View Article
human	WB,IHC	Eur J Med Res N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma Authors - Aierpati Maimaiti View Article
human	WB	Nat Struct Mol Biol Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer Authors - Yoichiro Sugimoto View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4E2 Polyclonal antibody

EIF4E2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 12227-1-AP

WB analysis of MCF-7 using 12227-1-AP

WB analysis of mouse kidney using 12227-1-AP

WB analysis of MCF-7 using 12227-1-AP

WB analysis of HeLa using 12227-1-AP

IP experiment of MCF-7 using 12227-1-AP

IHC staining of human breast cancer using 12227-1-AP

IHC staining of human cervical cancer using 12227-1-AP

IHC staining of human cervical cancer using 12227-1-AP

IHC staining of human breast cancer using 12227-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.

N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma

Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer