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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4EBP2 Polyclonal antibody

EIF4EBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11182-1-AP

Synonyms

Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using EIF4EBP2 Polyclonal antibody (11182-1-AP)

    WB analysis of mouse testis using 11182-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11182-1-AP (EIF4EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EIF4EBP2 Polyclonal antibody (11182-1-AP)

    IHC staining of human prostate cancer using 11182-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11182-1-AP (EIF4EBP2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11182-1-AP targets EIF4EBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4EBP2 fusion protein Ag1670 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E binding protein 2
Calculated molecular weight 13 kDa
Observed molecular weight ~20 kDa
GenBank accession numberBC050633
Gene symbol EIF4EBP2
Gene ID (NCBI) 1979
RRIDAB_2877750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
IHC protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Cell Mol Life Sci

Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs

Authors - Xiaolong Yuan
View Article