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  • Featured Product
  • KD/KO Validated

EIF4G1 Polyclonal antibody

EIF4G1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15704-1-AP

Synonyms

eIF-4G 1, EIF4G, EIF4F, eIF 4G1, eIF 4G 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4G1 Polyclonal antibody (15704-1-AP)

    WB analysis using 15704-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF4G1 Polyclonal antibody (15704-1-AP)

    WB analysis using 15704-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    WB analysis of HeLa using 15704-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    WB analysis of BxPC-3 using 15704-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    IP experiment of HeLa using 15704-1-AP

    IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF4G1 Polyclonal antibody (15704-1-AP)

    IHC staining of human breast cancer using 15704-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF4G1 Polyclonal antibody (15704-1-AP)

    IHC staining of human breast cancer using 15704-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    IF Staining of HepG2 using 15704-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4G1 antibody (15704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    IF Staining of HepG2 using 15704-1-AP

    Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4G1 Polyclonal antibody (15704-1-AP)

    IF Staining of HepG2 using 15704-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Ethacrynic acid treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15704-1-AP targets EIF4G1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8342 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular weight 1600 aa, 176 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC007788
Gene symbol EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2261979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
WB protocol for EIF4G1 antibody 15704-1-APDownload protocol
IHC protocol for EIF4G1 antibody 15704-1-APDownload protocol
IF protocol for EIF4G1 antibody 15704-1-APDownload protocol
IP protocol for EIF4G1 antibody 15704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
View Article
humanIF

J Extracell Vesicles

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.

Authors - Bo You
View Article
humanWB

Proc Natl Acad Sci U S A

Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.

Authors - Brian C Avanzino
View Article
humanWB

PLoS Genet

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

Authors - Siqi Hu
View Article
mouseWB,IF

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
  • KO Validated
View Article
humanWB

Virol Sin

Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A

Authors - Yanyan Dong
View Article