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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4G1 Monoclonal antibody, PBS Only

EIF4G1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B10G8

Cat no : 67199-1-PBS

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4G1 Monoclonal antibody (67199-1-Ig)

    WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using EIF4G1 Monoclonal antibody (67199-1-Ig)

    WB analysis of 4T1 using 67199-1-Ig (same clone as 67199-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67199-1-PBS targets EIF4G1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8357 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular weight 1600 aa, 176 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC007788
Gene symbol EIF4G1
Gene ID (NCBI) 1981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )