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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17728-1-AP

Synonyms

EIF4G2, Death-associated protein 5, Death associated protein 5, DAP-5, DAP5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis using 17728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis of HeLa using 17728-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis of MCF-7 using 17728-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IP experiment of MCF-7 using 17728-1-AP

    IP result of anti-eIF4G2/DAP5 (IP:17728-1-AP, 4ug; Detection:17728-1-AP 1:400) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IHC staining of human skin cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IHC staining of human skin cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IHC staining of human prostate cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IHC staining of human prostate cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IF Staining of HepG2 using 17728-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eIF4G2/DAP5 antibody (17728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, Neuro-2a cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17728-1-AP targets eIF4G2/DAP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12125 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated molecular weight 907 aa, 102 kDa
Observed molecular weight 98-102 kDa
GenBank accession numberBC043149
Gene symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2095895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IHC protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IF protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IP protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article
humanWB

Shock

miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2

Authors - Zirui Jia
View Article