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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis using 11281-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis of HEK-293 using 11281-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IP experiment of HeLa using 11281-1-AP

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using EIF4G3 Polyclonal antibody (11281-1-AP)

    IHC staining of human gliomas using 11281-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IF Staining of HeLa using 11281-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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EIF4G3 Polyclonal antibody

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11281-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11281-1-AP targets EIF4G3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4G3 fusion protein Ag1811 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 3
Calculated molecular weight 177 kDa
Observed molecular weight 70 kDa, 250 kDa
GenBank accession numberBC030578
Gene Symbol EIF4G3
Gene ID (NCBI) 8672
RRIDAB_2877754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43432
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for EIF4G3 antibody 11281-1-APDownload protocol
IHC protocol for EIF4G3 antibody 11281-1-APDownload protocol
IF protocol for EIF4G3 antibody 11281-1-APDownload protocol
IP protocol for EIF4G3 antibody 11281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Jpn J Clin Oncol

Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter.

Authors - Yan Huang
View Article
humanWB

Cancer Res

Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression

Authors - Lijie Zhou
View Article
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim
View Article