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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50372-1

    Sandwich ELISA standard curve of MP50372-1

    Sandwich ELISA standard curve of MP50372-1, Human EIF5A Monoclonal Matched Antibody Pair - PBS only. 67214-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1849. 67214-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL

  • Sample test of MP50372-1

    Sample test of MP50372-1

    The mean EIF5A concentration was determined to be 135.22 ng/mL in HEK-293 cell extract based on a 2.2 mg/mL extract load, 102.87 ng/mL in HeLa cell extract based on a 1.8 mg/mL extract load and 124.18 ng/mL in A549 cell extract based on a 3.1 mg/mL extract load.

  • Cytometric bead array standard curve of MP50372-1

    Cytometric bead array standard curve of MP50372-1

    Cytometric bead array standard curve of MP50372-1, EIF5A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67214-2-PBS. Detection antibody: 67214-3-PBS. Standard:Ag1849. Range: 1.563-50 ng/mL.

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EIF5A Monoclonal antibody, PBS Only (Capture)

EIF5A Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 67214-2-PBS
Clone No.1E3E10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67214-2-PBS targets EIF5A as part of a matched antibody pair:

MP50372-1: 67214-2-PBS capture and 67214-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF5A fusion protein Ag28588 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 5A
Calculated molecular weight 18 kDa
GenBank accession numberBC001832
Gene Symbol EIF5A
Gene ID (NCBI) 1984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP63241
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.