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EIF5B Polyclonal antibody
EIF5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13527-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
Product Information
13527-1-AP targets EIF5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EIF5B fusion protein Ag4404 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 5B |
| Calculated molecular weight | 1220 aa, 139 kDa |
| Observed molecular weight | 175 kDa |
| GenBank accession number | BC032639 |
| Gene symbol | EIF5B |
| Gene ID (NCBI) | 9669 |
| RRID | AB_2096200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF5B antibody 13527-1-AP | Download protocol |
| IHC protocol for EIF5B antibody 13527-1-AP | Download protocol |
| IF protocol for EIF5B antibody 13527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B. | ||
bioRxiv Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation
| ||
bioRxiv LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Bill (Verified Customer) (12-23-2019) | This antibody give every strong signal on EIF5B.
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