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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF6 Monoclonal antibody, PBS Only

EIF6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2A8

Cat no : 68765-3-PBS

Synonyms

integrin beta 4 binding protein, CAB, B4 integrin interactor, B(2)GCN homolog, b(2)gcn

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF6 Monoclonal antibody (68765-3-Ig)

    WB analysis using 68765-3-Ig (same clone as 68765-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68765-3-Ig (EIF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68765-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF6 Monoclonal antibody (68765-3-Ig)

    IF Staining of HeLa using 68765-3-Ig (same clone as 68765-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF6 antibody (68765-3-Ig, Clone: 1F2A8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68765-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68765-3-PBS targets EIF6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF6 fusion protein Ag30249 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 6
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001119
Gene symbol EIF6
Gene ID (NCBI) 3692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.