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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELA1 Polyclonal antibody (55342-1-AP)

    WB analysis using 55342-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55342-1-AP (ELA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ELA1 Polyclonal antibody

ELA1 Polyclonal Antibody for WB, ELISA
Cat No. 55342-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CELA1, ELA1, Elastase 1, elastase 1, pancreatic

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55342-1-AP targets ELA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name elastase 1, pancreatic
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberNM_001971
Gene Symbol ELA1
Gene ID (NCBI) 1990
RRIDAB_3086440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chymotrypsin-like elastase 1 (Cela1) is a digestive protease that is expressed during lung development in association with regions of elastin remodeling, exhibits stretch-dependent expression during lung regeneration, and binds lung elastin in a stretch-dependent manner..

Protocols

Product Specific Protocols
WB protocol for ELA1 antibody 55342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols