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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neutrophil Elastase Polyclonal antibody, PBS Only

Neutrophil Elastase Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27642-1-PBS

Synonyms

ELANE, ELA2, HNE, HLE, Elastase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neutrophil Elastase Polyclonal antibody (27642-1-AP)

    WB analysis using 27642-1-AP (same clone as 27642-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Neutrophil Elastase Polyclonal antibody (27642-1-AP)

    IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Neutrophil Elastase Polyclonal antibody (27642-1-AP)

    IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Neutrophil Elastase Polyclonal antibody (27642-1-AP)

    IHC staining of mouse spleen using 27642-1-AP (same clone as 27642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Neutrophil Elastase Polyclonal antibody (27642-1-AP)

    IF Staining of HEK-293 using 27642-1-AP (same clone as 27642-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27642-1-PBS targets Neutrophil Elastase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neutrophil Elastase fusion protein Ag26634 相同性解析による交差性が予測される生物種
Full Name elastase 2, neutrophil
Calculated molecular weight 267 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC074816
Gene symbol ELA2
Gene ID (NCBI) 1991
RRIDAB_2918126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.