Validation Data Gallery

Filter: AllWBIHC

WB analysis of DU 145 using 10071-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10071-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10071-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 10071-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10071-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ELAC2 Polyclonal antibody

ELAC2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10071-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, HeLa cells, HepG2 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IF	See 2 publications below

Product Information

10071-1-AP targets ELAC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ELAC2 fusion protein Ag0105 相同性解析による交差性が予測される生物種
Full Name	elaC homolog 2 (E. coli)
Calculated molecular weight	92 kDa
Observed molecular weight	92 kDa
GenBank accession number	BC004158
Gene Symbol	ELAC2
Gene ID (NCBI)	60528
RRID	AB_2096551
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BQ52
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

Protocols

Product Specific Protocols
WB protocol for ELAC2 antibody 10071-1-AP	Download protocol
IHC protocol for ELAC2 antibody 10071-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
human	IF	Cell Metab GRSF1 regulates RNA processing in mitochondrial RNA granules. Authors - Jourdain Alexis A AA View Article
human	WB	Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. Authors - Daniel F Bogenhagen View Article
human	WB	Nat Commun Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria. Authors - Zbigniew Pietras View Article
mouse	WB	Cell Rep Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly. Authors - Oliver Rackham View Article
human	WB,IF	RNA Biol Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs. Authors - Brzezniak Lien K LK KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of DU 145 using 10071-1-AP

WB analysis of HeLa using 10071-1-AP

WB analysis of HepG2 using 10071-1-AP

IHC staining of mouse testis using 10071-1-AP

IHC staining of mouse testis using 10071-1-AP

ELAC2 Polyclonal antibody

ELAC2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

GRSF1 regulates RNA processing in mitochondrial RNA granules.

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.

Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs.