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  • KD/KO Validated

ELAVL2 Polyclonal antibody

ELAVL2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14008-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inSH-SY5Y cells, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14008-1-AP targets ELAVL2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELAVL2 fusion protein Ag5105 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC030692
Gene Symbol ELAVL2
Gene ID (NCBI) 1993
RRIDAB_2096356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Protocols

Product Specific Protocols
WB protocol for ELAVL2 antibody 14008-1-APDownload protocol
IHC protocol for ELAVL2 antibody 14008-1-APDownload protocol
IP protocol for ELAVL2 antibody 14008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
mouseIP

Sci Adv

Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development

Authors - Yuki Takada
humanWB,IF,RIP

Nucleic Acids Res

HuB and HuD repress telomerase activity by dissociating HuR from TERC.

Authors - Xiaolei Cheng
  • KD Validated
mouseRIP

EMBO Rep

ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles.

Authors - Yuzuru Kato
chickenIF

Cell Rep

miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability.

Authors - Ronald J Parchem
humanWB

Hum Mol Genet

ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.

Authors - Stefano Berto
  • KD Validated
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