Validation Data Gallery
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Tested Applications
| Positive WB detected in | T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13107-1-AP targets ELF5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ELF5 fusion protein Ag3762 相同性解析による交差性が予測される生物種 |
| Full Name | E74-like factor 5 (ets domain transcription factor) |
| Calculated molecular weight | 255 aa, 30 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC029743 |
| Gene Symbol | ELF5 |
| Gene ID (NCBI) | 2001 |
| RRID | AB_3669152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKW6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELF5 is an epithelium-specific ETS family transcription factor. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ELF5 antibody 13107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |