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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of HeLa using 14666-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of MCF-7 using 14666-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of MCF-7 using 14666-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of MCF-7 using 14666-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of mouse liver using 14666-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of Jurkat using 14666-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of MCF-7 using 14666-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ELK4 Polyclonal antibody (14666-1-AP)

    WB analysis of HeLa using 14666-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ELK4 Polyclonal antibody

ELK4 Polyclonal Antibody for WB, ELISA
Cat No. 14666-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ELK4, SAP 1, SAP1, SRF accessory protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

14666-1-AP targets ELK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELK4 fusion protein Ag6273 相同性解析による交差性が予測される生物種
Full Name ELK4, ETS-domain protein (SRF accessory protein 1)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC063676
Gene Symbol ELK4
Gene ID (NCBI) 2005
RRIDAB_2262305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELK4, also named as SAP1, SAP-1 and SAP1, belongs to the ETS family. It forms a ternary complex with the serum response factor (SRF). ELK4 requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. It is androgen regulated, involved in promoting cell growth, and highly expressed in a subset of prostate cancer. (PMID:19293179 )

Protocols

Product Specific Protocols
WB protocol for ELK4 antibody 14666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway

Authors - Weiguo Xu
View Article
mouseWB

J Pers Med

Exosomes from Microvascular Endothelial Cells under Mechanical Unloading Inhibit Osteogenic Differentiation via miR-92b-3p/ELK4 Axis

Authors - Xiaoyan Zhang
View Article
mouseWB

Brain Res Bull

ELK4 ameliorates cognitive impairment and neuroinflammation induced by obstructive sleep apnea

Authors - Haiming Yang
View Article