Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ELL fusion protein Ag0617 相同性解析による交差性が予測される生物種 |
| Full Name | elongation factor RNA polymerase II |
| Calculated molecular weight | 500 aa, 68 kDa |
| Observed molecular weight | 68-80 kDa |
| GenBank accession number | BC049195 |
| Gene Symbol | ELL |
| Gene ID (NCBI) | 8178 |
| RRID | AB_2099137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55199 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ELL antibody 51044-1-AP | Download protocol |
| IHC protocol for ELL antibody 51044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.
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Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Prostate ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.
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Mol Immunol Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.
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