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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ELMOD2 Polyclonal antibody

ELMOD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13027-1-AP

Synonyms

9830169G11Rik, ELMOD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELMOD2 Polyclonal antibody (13027-1-AP)

    WB analysis using 13027-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13027-1-AP (ELMOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using ELMOD2 Polyclonal antibody (13027-1-AP)

    IP experiment of A549 using 13027-1-AP

    IP result of anti-ELMOD2 (IP:13027-1-AP, 4ug; Detection:13027-1-AP 1:300) with A549 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ELMOD2 Polyclonal antibody (13027-1-AP)

    IHC staining of human pancreas cancer using 13027-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ELMOD2 Polyclonal antibody (13027-1-AP)

    IHC staining of human pancreas cancer using 13027-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13027-1-AP targets ELMOD2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELMOD2 fusion protein Ag3653 相同性解析による交差性が予測される生物種
Full Name ELMO/CED-12 domain containing 2
Calculated molecular weight 293 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC015168
Gene symbol ELMOD2
Gene ID (NCBI) 255520
RRIDAB_2099579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ELMOD2 antibody 13027-1-APDownload protocol
IHC protocol for ELMOD2 antibody 13027-1-APDownload protocol
IP protocol for ELMOD2 antibody 13027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols