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  • Featured Product
  • KD/KO Validated

ELOVL5 Polyclonal antibody

ELOVL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26599-1-AP

Synonyms

ELOVL2, ELOVL5, Fatty acid elongase 5, HELO1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    WB analysis of HeLa using 26599-1-AP

    WB result of ELOVL5 antibody (26599-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELOVL5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ELOVL5 Polyclonal antibody (26599-1-AP)

    WB analysis using 26599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26599-1-AP (ELOVL5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ELOVL5 Polyclonal antibody (26599-1-AP)

    IHC staining of mouse liver using 26599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26599-1-AP (ELOVL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ELOVL5 Polyclonal antibody (26599-1-AP)

    IHC staining of mouse liver using 26599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26599-1-AP (ELOVL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    IF Staining of HeLa using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    IF Staining of HepG2 using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26599-1-AP targets ELOVL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELOVL5 fusion protein Ag24786 相同性解析による交差性が予測される生物種
Full Name ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight25 kDa
GenBank accession numberBC017270
Gene symbol ELOVL5
Gene ID (NCBI) 60481
RRIDAB_3669551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Very long-chain fatty acid elongase 5 (ELOVL5) is a key enzyme for the endogenous synthesis of long-chain unsaturated fatty acids. A critical role of ELOVL5 in elongating fatty acid substrates ranging from 16 to 20 carbons was reported in humans and rodents (PMID: 29454701). ELOVL5 is involved in metastasis through lipid droplets-regulated TGF-β receptor expression and is a predictive biomarker of metastatic ER+ breast cancer (PMID: 36056008). Western blot analysis detected ELOVL51 at an apparent molecular mass of ~25 kDa (PMID: 36056008, 32527375).

Protocols

Product Specific Protocols
WB protocol for ELOVL5 antibody 26599-1-APDownload protocol
IHC protocol for ELOVL5 antibody 26599-1-APDownload protocol
IF protocol for ELOVL5 antibody 26599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols