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  • Featured Product
  • KD/KO Validated

ELOVL5 Polyclonal antibody

ELOVL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26599-1-AP

Synonyms

ELOVL2, ELOVL5, Fatty acid elongase 5, HELO1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    WB analysis of HeLa using 26599-1-AP

    WB result of ELOVL5 antibody (26599-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELOVL5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ELOVL5 Polyclonal antibody (26599-1-AP)

    WB analysis using 26599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26599-1-AP (ELOVL5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ELOVL5 Polyclonal antibody (26599-1-AP)

    IHC staining of mouse liver using 26599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26599-1-AP (ELOVL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ELOVL5 Polyclonal antibody (26599-1-AP)

    IHC staining of mouse liver using 26599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26599-1-AP (ELOVL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    IF Staining of HeLa using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ELOVL5 Polyclonal antibody (26599-1-AP)

    IF Staining of HepG2 using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26599-1-AP targets ELOVL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELOVL5 fusion protein Ag24786 相同性解析による交差性が予測される生物種
Full Name ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight25 kDa
GenBank accession numberBC017270
Gene symbol ELOVL5
Gene ID (NCBI) 60481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Very long-chain fatty acid elongase 5 (ELOVL5) is a key enzyme for the endogenous synthesis of long-chain unsaturated fatty acids. A critical role of ELOVL5 in elongating fatty acid substrates ranging from 16 to 20 carbons was reported in humans and rodents (PMID: 29454701). ELOVL5 is involved in metastasis through lipid droplets-regulated TGF-β receptor expression and is a predictive biomarker of metastatic ER+ breast cancer (PMID: 36056008). Western blot analysis detected ELOVL51 at an apparent molecular mass of ~25 kDa (PMID: 36056008, 32527375).

Protocols

Product Specific Protocols
WB protocol for ELOVL5 antibody 26599-1-APDownload protocol
IHC protocol for ELOVL5 antibody 26599-1-APDownload protocol
IF protocol for ELOVL5 antibody 26599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols