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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EMCN Polyclonal antibody

EMCN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11828-1-AP

Synonyms

MUC-14, MUC14, Gastric cancer antigen Ga34, Endomucin-2, Endomucin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EMCN Polyclonal antibody (11828-1-AP)

    WB analysis using 11828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11828-1-AP (EMCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11828-1-AP targets EMCN in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMCN fusion protein Ag2396 相同性解析による交差性が予測される生物種
Full Name endomucin
Calculated molecular weight 19 kDa, 27 kDa
Observed molecular weight85 kDa
GenBank accession numberBC017781
Gene symbol EMCN
Gene ID (NCBI) 51705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endomucin (EMCN), also known as Endomucin 2 or Mucin 14, is a type I integral membrane glycoprotein that is endothelial-specific. EMCN is expressed on the surface of capillaries and venous and is highly expressed in highly vascularized tissues such as heart, kidney and lung (PMID: 29215001). Endomucin plays an important role in cell interaction, molecular cell signaling, angiogenesis and cell migration (PMID: 33532708). Since it is glycosylated, the apparent molecular weight of EMCN could be variable, ranging from 80 kDa to 120 kDa.

Protocols

Product Specific Protocols
WB protocol for EMCN antibody 11828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols