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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Emerin Recombinant antibody, PBS Only

Emerin Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230089B1

Cat no : 82888-1-PBS

Synonyms

EMD, STA, LEMD5, EDMD, 230089B1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Emerin Recombinant antibody (82888-1-RR)

    IHC staining of human stomach cancer using 82888-1-RR (same clone as 82888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-1-RR (Emerin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Emerin Recombinant antibody (82888-1-RR)

    IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Emerin Recombinant antibody (82888-1-RR)

    FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Emerin Recombinant antibody (82888-1-RR, Clone:230089B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82888-1-RR

    Affinity and Kinetic Characterization of 82888-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-1-RR against Human Emerin were performed. The affinity constant is 5.36 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82888-1-PBS targets Emerin in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Emerin fusion protein Ag0236 相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.