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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Emerin Recombinant antibody

Emerin Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230089C4

Cat no : 82888-2-RR

Synonyms

EDMD, EMD, emerin, LEMD5, STA

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EMD Recombinant antibody (82888-2-RR)

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EMD Recombinant antibody (82888-2-RR)

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EMD Recombinant antibody (82888-2-RR)

    IF Staining of HeLa using 82888-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity experiments of 82888-2-RR

    Affinity experiments of 82888-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-2-RR against Human Emerin were performed. The affinity constant is 1.71 nM.

Tested Applications

Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82888-2-RR targets Emerin in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Emerin fusion protein Ag0236 相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Emerin antibody 82888-2-RRDownload protocol
IF protocol for Emerin antibody 82888-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols