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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    WB analysis of human kidney using 10643-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    WB analysis of human placenta using 10643-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    WB analysis of human kidney using 10643-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    WB analysis of human brain using 10643-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human ovary tumor using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human colon using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10643-1-AP (EMILIN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human ovary using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human ovary using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human heart using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human heart using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human kidney using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human kidney using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human placenta using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human placenta using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human testis using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human testis using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human skin using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human skin using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human spleen using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human spleen using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human kidney using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human kidney using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human ovary tumor using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EMILIN1 Polyclonal antibody (10643-1-AP)

    IHC staining of human ovary tumor using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using EMILIN1 Polyclonal antibody (10643-1-AP)

    IF Staining of SKOV-3 using 10643-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using EMILIN1 antibody (10643-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

EMILIN1 Polyclonal antibody

EMILIN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10643-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Elastin microfibril interface-located protein 1, EMI, EMILIN, EMILIN 1, EMILIN-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue, human brain tissue, human placenta tissue
Positive IHC detected inhuman colon tissue, human heart tissue, human kidney tissue, human ovary tissue, human ovary tumor tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10643-1-AP targets EMILIN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMILIN1 fusion protein Ag1016 相同性解析による交差性が予測される生物種
Full Name elastin microfibril interfacer 1
Calculated molecular weight 107 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC007530
Gene Symbol EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2100192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6C2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EMILIN1, also named EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Protocols

Product Specific Protocols
WB protocol for EMILIN1 antibody 10643-1-APDownload protocol
IHC protocol for EMILIN1 antibody 10643-1-APDownload protocol
IF protocol for EMILIN1 antibody 10643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.

Authors - Genee Y Lee
View Article