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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EML1 Polyclonal antibody

EML1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12765-1-AP

Synonyms

HuEMAP-1, EMAP-1, EMAP, Echinoderm microtubule-associated protein-like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EML1 Polyclonal antibody (12765-1-AP)

    WB analysis using 12765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EML1 Polyclonal antibody (12765-1-AP)

    WB analysis of mouse brain using 12765-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using EML1 Polyclonal antibody (12765-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12765-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using EML1 Polyclonal antibody (12765-1-AP)

    IHC staining of human stomach using 12765-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12765-1-AP targets EML1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EML1 fusion protein Ag3429 相同性解析による交差性が予測される生物種
Full Name echinoderm microtubule associated protein like 1
Calculated molecular weight 815 aa, 90 kDa
Observed molecular weight 90-92 kDa
GenBank accession numberBC033043
Gene symbol EML1
Gene ID (NCBI) 2009
RRIDAB_10644132
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EML1 is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped.

Protocols

Product Specific Protocols
WB protocol for EML1 antibody 12765-1-APDownload protocol
IHC protocol for EML1 antibody 12765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF,WB

Front Cell Dev Biol

Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in Mice.

Authors - Hong Yin
View Article