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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EML4 Polyclonal antibody

EML4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22709-1-AP

Synonyms

Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    WB analysis of A549 using 22709-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EML4 Polyclonal antibody (22709-1-AP)

    WB analysis of HeLa using 22709-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using EML4 Polyclonal antibody (22709-1-AP)

    WB analysis of A431 using 22709-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IP experiment of A549 using 22709-1-AP

    IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human lung cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human lung cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human prostate cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human prostate cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EML4 antibody (22709-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22709-1-AP targets EML4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EML4 fusion protein Ag18463 相同性解析による交差性が予測される生物種
Full Name echinoderm microtubule associated protein like 4
Calculated molecular weight 981 aa, 109 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC140845
Gene symbol EML4
Gene ID (NCBI) 27436
RRIDAB_2879153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EML4 antibody 22709-1-APDownload protocol
IHC protocol for EML4 antibody 22709-1-APDownload protocol
IF protocol for EML4 antibody 22709-1-APDownload protocol
IP protocol for EML4 antibody 22709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols