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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENAH Polyclonal antibody

ENAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26421-1-AP

Synonyms

ENA, enabled homolog (Drosophila), ENAH, MENA, NDPP1, Protein enabled homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using ENAH Polyclonal antibody (26421-1-AP)

    WB analysis of U-251 using 26421-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ENAH Polyclonal antibody (26421-1-AP)

    WB analysis using 26421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ENAH Polyclonal antibody (26421-1-AP)

    WB analysis of MCF-7 using 26421-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ENAH Polyclonal antibody (26421-1-AP)

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ENAH Polyclonal antibody (26421-1-AP)

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ENAH Polyclonal antibody (26421-1-AP)

    IF Staining of MCF-7 using 26421-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ENAH antibody (26421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA431 cells, U-251 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26421-1-AP targets ENAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENAH fusion protein Ag24200 相同性解析による交差性が予測される生物種
Full Name enabled homolog (Drosophila)
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 85-88 kDa, 75-80 kDa
GenBank accession numberBC095481
Gene symbol ENAH
Gene ID (NCBI) 55740
RRIDAB_2880509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ENAH antibody 26421-1-APDownload protocol
IHC protocol for ENAH antibody 26421-1-APDownload protocol
IF protocol for ENAH antibody 26421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols