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Endoglin/CD105 Polyclonal antibody
Endoglin/CD105 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10862-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, human placenta tissue, mouse lung tissue, rat lung tissue |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human breast cancer tissue, rat kidney tissue, human placenta tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 14 publications below |
IHC | See 9 publications below |
IF | See 12 publications below |
FC | See 3 publications below |
Product Information
10862-1-AP targets Endoglin/CD105 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Endoglin/CD105 fusion protein Ag1287 相同性解析による交差性が予測される生物種 |
Full Name | endoglin |
Calculated molecular weight | 71 kDa |
Observed molecular weight | 75-90 kDa, 170-180 kDa |
GenBank accession number | BC014271 |
Gene symbol | CD105 |
Gene ID (NCBI) | 2022 |
RRID | AB_2098906 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
Protocols
Product Specific Protocols | |
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WB protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
IHC protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
IP protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Dev Cell Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation | ||
Cancers (Basel) WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells. | ||
Int J Biol Sci Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA. | ||
Biomedicines Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.
| ||
Mol Hum Reprod Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells. | ||
World J Stem Cells Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis |