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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endoglin/CD105 Monoclonal antibody, PBS Only (Detector)

Endoglin/CD105 Monoclonal Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4A5

Cat no : 68748-2-PBS

Synonyms

CD105, 2D4A5, HHT1, ENG, Endoglin

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Endoglin/CD105 Monoclonal antibody (68748-2-Ig)

    IF Staining of HUVEC using 68748-2-Ig (same clone as 68748-2-PBS)

    Immunofluorescent analysis of un-fixed HUVEC cells using Endoglin/CD105 antibody (68748-2-Ig, Clone: 2D4A5 ) at dilution of 1:1422 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68748-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50103-1

    Cytometric bead array standard curve of MP50103-1

    Cytometric bead array standard curve of MP50103-1, ENG Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68748-1-PBS. Detection antibody: 68748-2-PBS. Standard:Eg0587. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68748-2-PBS targets Endoglin/CD105 as part of a matched antibody pair:

MP50103-1: 68748-1-PBS capture and 68748-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)