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  • Featured Product
  • KD/KO Validated

ENO1/2/3 Recombinant antibody, PBS Only

ENO1/2/3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E24

Cat no : 81504-1-PBS

Synonyms

ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENO1/2/3 Recombinant antibody (81504-1-RR)

    WB analysis using 81504-1-RR (same clone as 81504-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ENO1/2/3 Recombinant antibody (81504-1-RR)

    WB analysis of HEK-293 using 81504-1-RR (same clone as 81504-1-PBS)

    WB result of ENO1/2/3 antibody (81504-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1/2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENO1/2/3 Recombinant antibody (81504-1-RR)

    IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENO1/2/3 Recombinant antibody (81504-1-RR)

    IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ENO1/2/3 Recombinant antibody (81504-1-RR)

    IHC staining of human colon cancer using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ENO1/2/3 Recombinant antibody (81504-1-RR)

    IHC staining of human colon cancer using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ENO1/2/3 Recombinant antibody (81504-1-RR)

    IF Staining of HepG2 using 81504-1-RR (same clone as 81504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1/2/3 antibody (81504-1-RR, Clone: 6E24 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81504-1-PBS targets ENO1/2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENO1/2/3 fusion protein Ag1692 相同性解析による交差性が予測される生物種
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC015641
Gene symbol ENO1
Gene ID (NCBI) 2023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.