Featured Product
KD/KO Validated

ENO1 Polyclonal antibody

Name: ENO1 Polyclonal antibody
Brand: Proteintech
SKU: 55237-1-AP

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55237-1-AP

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 55237-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55237-1-AP
WB result of ENO1 antibody (55237-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.
WB analysis of mouse brain using 55237-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55237-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55237-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55237-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55237-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 55237-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 55237-1-AP
IP result of anti-ENO1 (IP:55237-1-AP, 4ug; Detection:55237-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human brain using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skeletal muscle using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas using 55237-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 55237-1-AP
Immunofluorescent analysis of HepG2 cells using 55237-1-AP (ENO1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

-/ -

Cat No. 55237-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, HepG2 cells, mouse liver tissue, HEK-293 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human brain tissue, human pancreas tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IP	See 1 publications below

Product Information

55237-1-AP targets ENO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	enolase 1, (alpha)
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	NM_001428
Gene symbol	ENO1
Gene ID (NCBI)	2023
RRID	AB_10837362
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross reaction with ENO2 and ENO3.

Protocols

Product Specific Protocols
WB protocol for ENO1 antibody 55237-1-AP	Download protocol
IHC protocol for ENO1 antibody 55237-1-AP	Download protocol
IF protocol for ENO1 antibody 55237-1-AP	Download protocol
IP protocol for ENO1 antibody 55237-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	EMBO Rep Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis. Authors - Guang Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 55237-1-AP

WB analysis of HEK-293 using 55237-1-AP

WB analysis of mouse brain using 55237-1-AP

WB analysis of mouse brain using 55237-1-AP

WB analysis of HeLa using 55237-1-AP

WB analysis of HepG2 using 55237-1-AP

WB analysis of HepG2 using 55237-1-AP

WB analysis of mouse liver using 55237-1-AP

IP experiment of mouse brain using 55237-1-AP

IHC staining of human brain using 55237-1-AP

IHC staining of human brain using 55237-1-AP

IHC staining of human brain using 55237-1-AP

IHC staining of human brain using 55237-1-AP

IHC staining of human skeletal muscle using 55237-1-AP

IHC staining of human skeletal muscle using 55237-1-AP

IHC staining of human pancreas using 55237-1-AP

IHC staining of human pancreas using 55237-1-AP

IHC staining of human pancreas using 55237-1-AP

IHC staining of human pancreas using 55237-1-AP

IF Staining of HepG2 using 55237-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.