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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENO1 Recombinant antibody (81478-1-RR)

    WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENO1 Recombinant antibody (81478-1-RR)

    WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ENO1 Recombinant antibody (81478-1-RR)

    WB analysis of HEK-293 using 81478-1-RR (same clone as 81478-1-PBS)

    WB result of ENO1 antibody (81478-1-RR; 1:300000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ENO1 Recombinant antibody (81478-1-RR)

    WB analysis of pig brain using 81478-1-RR (same clone as 81478-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENO1 Recombinant antibody (81478-1-RR)

    IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENO1 Recombinant antibody (81478-1-RR)

    IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ENO1 Recombinant antibody (81478-1-RR)

    IHC staining of human colon cancer using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ENO1 Recombinant antibody (81478-1-RR)

    IHC staining of human colon cancer using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ENO1 Recombinant antibody (81478-1-RR)

    IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (81478-1-RR, Clone: 2C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81478-1-RR

    Affinity and Kinetic Characterization of 81478-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81478-1-RR against Human ENO1 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

ENO1 Recombinant antibody, PBS Only

ENO1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81478-1-PBS
Clone No.2C20

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81478-1-PBS targets ENO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENO1 fusion protein Ag1692 相同性解析による交差性が予測される生物種
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06733
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.