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  • Featured Product
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NSE/ENO2-specific Polyclonal antibody

NSE/ENO2-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55235-1-AP

Synonyms

Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    WB analysis of U-251 using 55235-1-AP

    WB result of NSE/ENO2-specific antibody (55235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE/ENO2-specific transfected U-251 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    WB analysis of SH-SY5Y using 55235-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    WB analysis of HepG2 using 55235-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    WB analysis of mouse brain using 55235-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    WB analysis of HEK-293 using 55235-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human brain using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human brain using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human stomach cancer using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human stomach cancer using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human stomach cancer using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NSE/ENO2-specific Polyclonal antibody (55235-1-AP)

    IHC staining of human stomach cancer using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, HEK-293 cells, HepG2 cells, U-251 cells
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55235-1-AP targets NSE/ENO2-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name enolase 2 (gamma, neuronal)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_001975
Gene symbol NSE
Gene ID (NCBI) 2026
RRIDAB_10863290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. This antibody is specific to ENO2 and has no cross reaction to ENO1 and ENO3.

Protocols

Product Specific Protocols
WB protocol for NSE/ENO2-specific antibody 55235-1-APDownload protocol
IHC protocol for NSE/ENO2-specific antibody 55235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.

Authors - Chunli Xu
View Article