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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENO3 Polyclonal antibody, PBS Only

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16421-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human skeletal muscle using 16421-1-AP (same clone as 16421-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human brain using 16421-1-AP (same clone as 16421-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENO3 Polyclonal antibody (16421-1-AP)

    IF Staining of HeLa using 16421-1-AP (same clone as 16421-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16421-1-AP (ENO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16421-1-PBS targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENO3 fusion protein Ag9613 相同性解析による交差性が予測される生物種
Full Name enolase 3 (beta, muscle)
Calculated molecular weight 434 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC017249
Gene symbol ENO3
Gene ID (NCBI) 2027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).