Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68147-1-Ig targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ENO3 fusion protein Ag9642 相同性解析による交差性が予測される生物種 |
| Full Name | enolase 3 (beta, muscle) |
| Calculated molecular weight | 434 aa, 47 kDa |
| Observed molecular weight | 42-47 kDa |
| GenBank accession number | BC017249 |
| Gene Symbol | ENO3 |
| Gene ID (NCBI) | 2027 |
| RRID | AB_2923671 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ENO3 antibody 68147-1-Ig | Download protocol |
| IF protocol for ENO3 antibody 68147-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |