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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENOSF1 Polyclonal antibody (31022-1-AP)

    WB analysis using 31022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ENOSF1 Polyclonal antibody (31022-1-AP)

    IP experiment of MCF-7 using 31022-1-AP

    IP result of anti-ENOSF1 (IP:31022-1-AP, 4ug; Detection:31022-1-AP 1:2000) with MCF-7 cells lysate 1600 ug.

  • Flow cytometry (FC) experiment of HeLa cells using ENOSF1 Polyclonal antibody (31022-1-AP)

    FC experiment of HeLa using 31022-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ENOSF1 Polyclonal antibody (31022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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ENOSF1 Polyclonal antibody

ENOSF1 Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 31022-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31022-1-AP targets ENOSF1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENOSF1 fusion protein Ag34665 相同性解析による交差性が予測される生物種
Full Name enolase superfamily member 1
Calculated molecular weight 50 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC001285
Gene Symbol ENOSF1
Gene ID (NCBI) 55556
RRIDAB_3669818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7L5Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

Protocols

Product Specific Protocols
WB protocol for ENOSF1 antibody 31022-1-APDownload protocol
IP protocol for ENOSF1 antibody 31022-1-APDownload protocol
FC protocol for ENOSF1 antibody 31022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols