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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENOSF1 Recombinant antibody (83769-2-RR)

    WB analysis using 83769-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Hela cells using ENOSF1 Recombinant antibody (83769-2-RR)

    FC experiment of Hela using 83769-2-RR

    1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83769-2-RR

    Affinity and Kinetic Characterization of 83769-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

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ENOSF1 Recombinant antibody

ENOSF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83769-2-RR
Clone No.240796D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83769-2-RR targets ENOSF1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENOSF1 fusion protein Ag34665 相同性解析による交差性が予測される生物種
Full Name enolase superfamily member 1
Calculated molecular weight 50 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC001285
Gene Symbol ENOSF1
Gene ID (NCBI) 55556
RRIDAB_3671363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L5Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

Protocols

Product Specific Protocols
WB protocol for ENOSF1 antibody 83769-2-RRDownload protocol
FC protocol for ENOSF1 antibody 83769-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols