ENPP1 antibody (14011-1-AP) | Proteintech

Validation Data Gallery

Filter: AllWBIP

WB analysis of Raji using 14011-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14011-1-AP (ENPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 14011-1-AP
IP result of anti-ENPP1 (IP:14011-1-AP, 4ug; Detection:14011-1-AP 1:1000) with Raji cells lysate 3200 ug.

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Tested Applications
Product Information

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Tested Applications

Positive WB detected in	Raji cells
Positive IP detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3200
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14011-1-AP targets ENPP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ENPP1 fusion protein Ag5117 相同性解析による交差性が予測される生物種
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase 1
Calculated molecular weight	105 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC059375
Gene Symbol	ENPP1
Gene ID (NCBI)	5167
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22413
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.