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ENPP2 Polyclonal antibody

ENPP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14243-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

ATX, ATX X, Autotaxin, E NPP 2, ENPP2, LysoPLD, PD IALPHA, PDNP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue, mouse kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14243-1-AP targets ENPP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENPP2 fusion protein Ag5499 相同性解析による交差性が予測される生物種
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 2
Calculated molecular weight 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC034961
Gene Symbol ENPP2
Gene ID (NCBI) 5168
RRIDAB_2878033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13822
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Protocols

Product Specific Protocols
WB protocol for ENPP2 antibody 14243-1-APDownload protocol
IHC protocol for ENPP2 antibody 14243-1-APDownload protocol
IP protocol for ENPP2 antibody 14243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.

Authors - Jillian A Bristol
human,mouseWB

Cells

Role of Aberrantly Activated Lysophosphatidic Acid Receptor 1 Signaling Mediated Inflammation in Renal Aging.

Authors - Yongjie Jin
mouseWB

Front Pharmacol

UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar.

Authors - Fangbo Xia
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
mouseWB

Am J Physiol Cell Physiol

The Ecto-Nucleotide Pyrophosphatase/Phosphodiesterase 2 Promotes Early Osteoblast Differentiation and Mineralization in Stromal Stem Cells

Authors - Irina L Tourkova
mouseWB

Thyroid

Increased thyroid hormone action alleviates hippocampal damage by down-regulating neuronal type-Ⅰ interferon signaling/necroptosis in diabetes-associated cognitive dysfunction

Authors - Ling Tian
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