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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENSA Polyclonal antibody

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14518-1-AP

Synonyms

ARPP-19e, Alpha-endosulfine

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis using 14518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of mouse brain using 14518-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of HeLa using 14518-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human small intestine using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    IF Staining of HeLa using 14518-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14518-1-AP (ENSA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, HeLa cells
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14518-1-AP targets ENSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENSA fusion protein Ag5982 相同性解析による交差性が予測される生物種
Full Name endosulfine alpha
Calculated molecular weight 14 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC004461
Gene symbol ENSA
Gene ID (NCBI) 2029
RRIDAB_10838808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.

Protocols

Product Specific Protocols
WB protocol for ENSA antibody 14518-1-APDownload protocol
IHC protocol for ENSA antibody 14518-1-APDownload protocol
IF protocol for ENSA antibody 14518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols