CD39/ENTPD1 Polyclonal antibody

Name: CD39/ENTPD1 Polyclonal antibody
Brand: Proteintech
SKU: 14211-1-AP

CD39/ENTPD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14211-1-AP

Synonyms

ENTPD1, Ecto apyrase, EC:3.6.1.5, CD39, ATP-DPH

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICC

WB analysis of mouse placenta using 14211-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (ENTPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14211-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14211-1-AP
human placenta lysates were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 14211-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 14211-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD39/ENTPD1 antibody (14211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14211-1-AP
Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14211-1-AP
Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 14211-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse placenta tissue, human placenta tissue
Positive IHC detected in	human placenta tissue, human tonsillitis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

14211-1-AP targets CD39/ENTPD1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD39/ENTPD1 fusion protein Ag5429 相同性解析による交差性が予測される生物種
Full Name	ectonucleoside triphosphate diphosphohydrolase 1
Calculated molecular weight	58 kDa
Observed molecular weight	50 kDa，70-100 kDa
GenBank accession number	BC047664
Gene symbol	CD39
Gene ID (NCBI)	953
RRID	AB_10638609
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 14211-1-AP	Download protocol
IHC protocol for CD39/ENTPD1 antibody 14211-1-AP	Download protocol
IF protocol for CD39/ENTPD1 antibody 14211-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	Int J Biol Sci CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence Authors - Zhenni Liu View Article
human	IF	Genomics Proteomics Bioinformatics Single-cell Immune Landscape of Human Recurrent Miscarriage. Authors - Feiyang Wang View Article
human	IHC	Oncotarget Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation. Authors - Federica Costa View Article
rat	IHC	Am J Physiol Gastrointest Liver Physiol Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity. Authors - Moyuru Hayashi View Article
human	WB	BMC Med Genomics Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis. Authors - Xing-Cheng Zhao View Article
rat	WB	J Gerontol A Biol Sci Med Sci Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling Authors - Lori A Birder View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Validation Data Gallery

WB analysis of mouse placenta using 14211-1-AP

WB analysis of human placenta using 14211-1-AP

WB analysis using 14211-1-AP

IHC staining of human placenta using 14211-1-AP

IHC staining of human tonsillitis using 14211-1-AP

IHC staining of human placenta using 14211-1-AP

IHC staining of human tonsillitis using 14211-1-AP

IHC staining of human placenta using 14211-1-AP

IHC staining of human placenta using 14211-1-AP

IHC staining of human liver using 14211-1-AP

IHC staining of human liver using 14211-1-AP

IHC staining of human tonsillitis using 14211-1-AP

IF Staining of human placenta using 14211-1-AP

IF Staining of HeLa using 14211-1-AP

IF Staining of HeLa using 14211-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence

Single-cell Immune Landscape of Human Recurrent Miscarriage.

Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.

Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling