Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, Jurkat cells, Raji cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
13021-1-AP targets ENTPD3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ENTPD3 fusion protein Ag4114 相同性解析による交差性が予測される生物種 |
| Full Name | ectonucleoside triphosphate diphosphohydrolase 3 |
| Calculated molecular weight | 529 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC029869 |
| Gene Symbol | ENTPD3 |
| Gene ID (NCBI) | 956 |
| RRID | AB_2100082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ENTPD3 antibody 13021-1-AP | Download protocol |
| IF protocol for ENTPD3 antibody 13021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice. | ||
Reprod Sci Expression and Hormonal Regulation of Entpd3 at Various Estrous Cycle Stages in the Mouse Uterus |