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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ENY2 Polyclonal antibody (15778-1-AP)

    WB analysis of PC-3 using 15778-1-AP (same clone as 15778-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15778-1-AP (ENY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ENY2 Polyclonal antibody (15778-1-AP)

    IHC staining of human breast cancer using 15778-1-AP (same clone as 15778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15778-1-AP (ENY2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ENY2 Polyclonal antibody (15778-1-AP)

    IHC staining of human breast cancer using 15778-1-AP (same clone as 15778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15778-1-AP (ENY2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15778-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ENY2 Polyclonal antibody, PBS Only

ENY2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15778-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15778-1-PBS targets ENY2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENY2 fusion protein Ag8462 相同性解析による交差性が予測される生物種
Full Name enhancer of yellow 2 homolog (Drosophila)
Calculated molecular weight 12 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC007870
Gene Symbol ENY2
Gene ID (NCBI) 56943
RRIDAB_2878184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPA8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ENY2, also named as DC6, is involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. It is required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). The MW of ENY2 is about 10 kDa.