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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EOMES/TBR2 Recombinant antibody

EOMES/TBR2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241036D9

Cat no : 83945-5-RR

Synonyms

EOMES, TBR2, 241036D9, T-box brain protein 2, T brain 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EOMES Recombinant antibody (83945-5-RR)

    WB analysis using 83945-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83945-5-RR (EOMES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using EOMES Recombinant antibody (83945-5-RR)

    IF Staining of SH-SY5Y using 83945-5-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using EOMES antibody (83945-5-RR, Clone: 241036D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83945-5-RR

    Affinity and Kinetic Characterization of 83945-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human EOMES were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inNK-92 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83945-5-RR is EOMES/TBR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EOMES/TBR2 fusion protein Ag27634 相同性解析による交差性が予測される生物種
Full Name eomesodermin homolog (Xenopus laevis)
Calculated molecular weight 73 aa
Observed molecular weight 67 kDa
GenBank accession numberNM_001278182
Gene symbol EOMES
Gene ID (NCBI) 8320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).

Protocols

Product Specific Protocols
WB protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
IF protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols