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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p300 Polyclonal antibody

p300 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20695-1-AP

Synonyms

EP300, Histone crotonyltransferase p300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of mouse lung using 20695-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of mouse lung using 20695-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of rat lung using 20695-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293T cells, Jurkat cells, PC-3 cells
Positive IHC detected inrat lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20695-1-AP targets p300 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001429
Gene symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Protocols

Product Specific Protocols
WB protocol for p300 antibody 20695-1-APDownload protocol
IHC protocol for p300 antibody 20695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Targeting gut microbial nitrogen recycling and cellular uptake of ammonium to improve bortezomib resistance in multiple myeloma

Authors - Yinghong Zhu
View Article