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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EP300 Recombinant antibody, PBS Only (Capture)

EP300 Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230369H8

Cat no : 83078-4-PBS

Synonyms

p300, Histone crotonyltransferase p300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p300 Recombinant antibody (83078-4-RR)

    IF Staining of HepG2 using 83078-4-RR (same clone as 83078-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using p300 Recombinant antibody (83078-4-RR)

    FC experiment of THP-1 using 83078-4-RR (same clone as 83078-4-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2, EP300 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83078-4-PBS. Detection antibody: 83078-3-PBS. Standard: SY00911. Range: 0.313-5 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83078-4-PBS targets EP300 as part of a matched antibody pair:

MP00310-2: 83078-4-PBS capture and 83078-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
GenBank accession numberNM_001429
Gene symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.